P-131: Autologous Transplantation Encapsulated Preantral Follicles into Fibrin Hydrogel Supplemented with Platelet Lysate

Background: Recent improvement in treatments of cancer has significantly increased survival rates of cancer patients. Unfortunately these treatments such as chemotherapy and radiotherapy can sever damage to the ovaries. Ovarian cryopreservation prior to chemo/ radiotherapy and auto-transplantation post-treatment can restore fertility to cancer patients. Hypoxia in the transplant from delayed revascularization is one of the concerns about this strategy. Thus, we assumed that isolated follicles transplantation with fibrin hydrogel supplemented platelet lysate (PL) lessen this concern. Materials and Methods: In this study NMRI mice (4 weeks) heterotopic grafted with own follicles. Follicles were mechanically isolated using insulin-gauge needles and encapsulated into fibrin hydrogel droplets supplemented with PL 15%. Follicles with centrally located oocytes and two layers of granulosa cells (100-130 μm in diameter) were selected. In this study a total of 844 follicles were divided into two groups: A. 389 follicles encapsulated into fibrin hydrogel B. 455 follicles encapsulated into fibrin hydrogel supplemented PL 15%, Then implanted heterotopic under the neck skin (hypoderm) for a period of 14 days. Finally, survival, growth and number of grafted follicles were being assessment with Hematoxylin and Eosin staining and histological sections were analyzed to determine follicle number and stage. Results: We observed in group A (encapsulated follicles into fibrin without PL supplement) from 389 transplanted follicles 115 follicles survived (survival rate 29.56%) that 78 follicles were in pre-antral stage (more than 2-3 layers of granulosa cells, pre-antral rate 67.82%) and 37 follicles were in antral stage (antral rate 32.17%). In group B (encapsulated follicles into fibrin supplemented PL 15%) from 455 transplanted follicles 178 follicles survived (survival rate 39.12%) that 122 follicles were in pre-antral stage (more than 2-3 layers of granulosa cells, pre-antral rate 68.53%) and 56 follicles were in antral stage (antral rate 31.81%). Conclusion: Our results demonstrate the autologous transplantation encapsulated pre-antral follicles into fibrin hydrogel supplemented platelet lysate improves survival rate and folliculogenesis. Also fibrin hydrogel matrix as biodegradable scaffold physically bridges the transplant and host tissue, and platelet lysate as a growth and angiogenic rich product would promote revascularization and follicle survival.